The tests were led by Dr. Val Flechtner, Ph.D Biologist at John Carroll University.

Methodology:

1. Subjects running their fingers through their hair to make sure there were bacteria present.
2. Subjects pressing three fingers of one hand against the surface of the agar plate.
3. Subjects directed to use the sanitizer as they would normally use a sanitizer.
4. Students pressing the same three fingers of their hand against the surface of a new plate.

To make sure the Petri dishes were not contaminated, plates were incubated at 37 C 40-44 hours. They were examined visually for growth of bacteria. All plates were used within five days of preparation. They were stored in sealed plastic bags prior to use.

There was no wholesale contamination of this batch of plates. No bacterial growth was noted on plates held 10 days at room temperature.

One could see that the growth of bacteria on both the before and after plates occurred in three clusters which corresponded to the imprint on the students fingers on the surface of the plate. In addition, two trials were run for each product; the results between all tests for each product were consistent.

Here are the results of the tests:

CONTROL BATCH:

• Control batch of 64 percent Ethyl Alcohol –- showed significant reduction of bacteria count after use.
• Control batch of water -– showed NO reduction of bacteria count after washing hands with water.
• Control batch of 64 percent Ethyl Alcohol with glycerin –- showed significant reduction of bacteria count after use.

PRODUCTS:

• Lucky Hand Sanitizer -- showed little to no reduction of bacteria count after use.
• Sani-Doo Hand Sanitizer -- showed little to no reduction of bacteria count after use.
• April Bath & Shower Hand Sanitizer –- showed little to no reduction of bacteria count after use.
• American Red Cross -– showed significant reduction of bacteria count after use.
• Purell -- showed significant reduction of bacteria count after use.
• "Pure" Generic Hand Sanitizer -- showed little to no reduction of bacteria count after use.
• Family Dollar Generic Hand Sanitizer -– showed little to no reduction of bacteria count after use.
• Target Generic Hand Sanitizer -- showed significant reduction of bacteria count after use.
• CVS Generic Hand Sanitizer -- showed significant reduction of bacteria count after use.
• Walgreens Generic Hand Sanitizer -- showed significant reduction of bacteria count after use.
• Rite Aid Generic Hand Sanitizer -- showed some reduction of bacteria count after use.
• Personal Care Hand Sanitizer -- showed little to no reduction of bacteria count after use.